Prohibitin antibody [5H7]

Cat. No. GTX60495

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	5H7
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat, Monkey
Package	100 μl ($409)

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View product citations for antibody GTX60495 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PHB expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887975

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prohibitin , HEL-215 , HEL-S-54e , PHB1

Cellular Localization

Mitochondrion inner membrane

Background

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Gene ID: 5245 PHB
UniProt: P35232 PHB

Research Area

DATA IMAGES

GTX60495 IHC-P Image

IHC-P analysis of stomach cancer tissue using GTX60495 Prohibitin antibody [5H7].

GTX60495 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60495 Prohibitin antibody [5H7].

GTX60495 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60495 Prohibitin antibody [5H7].

GTX60495 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60495 Prohibitin antibody [5H7].

GTX60495 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX60495 Prohibitin antibody [5H7].
Green : Prohibitin
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60495 WB Image

WB analysis of human Prohibitin (AA: 68-259) recombinant protein using GTX60495 Prohibitin antibody [5H7].

GTX60495 WB Image

WB analysis of A431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A549 (6), NIH3T3 (7), Cos7 (8) and PC-12 (9) cell lysate using GTX60495 Prohibitin antibody [5H7].

GTX60495 ELISA Image

ELISA analysis of antigen using GTX60495 Prohibitin antibody [5H7].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60495 FACS Image

FACS analysis of MCF-7 cells using GTX60495 Prohibitin antibody [5H7].
Blue : Prohibitin
Red : negative control

REFERENCE

Application Reference

Buehler U et al. EMBO J 2018; 37 (16) Targeting prohibitins at the cell surface prevents Th17-mediated autoimmunity.

Application : FACS

Reactivity : Mouse

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REVIEW

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Submit a Review

Application Reference

Buehler U et al. EMBO J 2018; 37 (16) Targeting prohibitins at the cell surface prevents Th17-mediated autoimmunity.

Application : FACS

Reactivity : Mouse

SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty

Prohibitin antibody [5H7]

Cat. No. GTX60495

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX60495 IHC-P Image

GTX60495 IHC-P Image

GTX60495 IHC-P Image

GTX60495 IHC-P Image

GTX60495 ICC/IF Image

GTX60495 WB Image

GTX60495 WB Image

GTX60495 ELISA Image

GTX60495 FACS Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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