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RUNX1 antibody

Orthogonal
Anti-RUNX1 antibody used in Western Blot (WB). GTX129100
Anti-RUNX1 antibody used in Western Blot (WB). GTX129100
Anti-RUNX1 antibody used in Western Blot (WB). GTX129100
Anti-RUNX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129100

Cat. No. GTX129100

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RUNX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Raw264.7 , C2C12 , Rat2 , Jurkat nuclear extract

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUNX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885895

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

runt related transcription factor 1 , AML1 , AML1-EVI-1 , AMLCR1 , CBF2alpha , CBFA2 , EVI-1 , PEBP2aB , PEBP2alpha

Cellular Localization

Nucleus

Background

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RUNX1 antibody used in Western Blot (WB). GTX129100

GTX129100 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody (GTX129100) diluted at 1:1000.

Anti-RUNX1 antibody used in Western Blot (WB). GTX129100

GTX129100 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody (GTX129100) diluted at 1:1000.

Anti-RUNX1 antibody used in Western Blot (WB). GTX129100

GTX129100 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody (GTX129100) diluted at 1:1000.

Anti-RUNX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129100

GTX129100 ICC/IF Image

RUNX1 antibody detects RUNX1 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUNX1 protein stained by RUNX1 antibody (GTX129100) diluted at 1:400.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE

Application Reference

Ackerman WE 4th et al. bioRxiv 2024; Epigenetic changes regulating the epithelial-mesenchymal transition in human trophoblast differentiation.
Liu Y et al. Cell Cycle 2019; 18 (4) : 437-451 Atypical GATA protein TRPS1 plays indispensable roles in mouse two-cell embryo.
Application : IHC
Reactivity : Mouse
Lappas M et al. Mol Cell Endocrinol 2018; (Epub) Runt-related transcription factor 1 (RUNX1) deficiency attenuates inflammation-induced pro-inflammatory and pro-labour mediators in myometrium.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169