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SLU7 antibody

Anti-SLU7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125955
Anti-SLU7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125955
Anti-SLU7 antibody used in Western Blot (WB). GTX125955
Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125955

Cat. No. GTX125955

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SLU7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SLU7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885618

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SLU7 homolog, splicing factor , 9G8 , hSlu7

Cellular Localization

Nucleus , Cytoplasm

Background

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SLU7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125955

GTX125955 ICC/IF Image

Slu7 antibody detects Slu7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Slu7 protein stained by Slu7 antibody (GTX125955) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SLU7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125955

GTX125955 ICC/IF Image

Slu7 antibody detects Slu7 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Slu7 protein stained by Slu7 antibody (GTX125955) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SLU7 antibody used in Western Blot (WB). GTX125955

GTX125955 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX125955 diluted at 1:5000

Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125955

GTX125955 IHC-P Image

Slu7 antibody detects SLU7 protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
Slu7 antibody (GTX125955) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169