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WRN antibody

Anti-WRN antibody used in IHC (Paraffin sections) (IHC-P). GTX101081
Anti-WRN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101081
Anti-WRN antibody used in Western Blot (WB). GTX101081

Cat. No. GTX101081

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all WRN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

162 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WRN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038249

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Werner syndrome RecQ like helicase , RECQ3 , RECQL2 , RECQL3

Cellular Localization

Nucleus , nucleolus

Background

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-WRN antibody used in IHC (Paraffin sections) (IHC-P). GTX101081

GTX101081 IHC-P Image

WRN antibody detects WRN protein at nucleus on human normal colon by immunohistochemical analysis.
Sample: Paraffin-embedded human normal colon.
WRN antibody (GTX101081) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WRN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101081

GTX101081 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using WRN(GTX101081) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-WRN antibody used in Western Blot (WB). GTX101081

GTX101081 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
5% SDS PAGE
GTX101081 diluted at 1:1000

REFERENCE

Application Reference

Kang SM et al. Sci Rep 2021; 11 (1) : 9122 Human WRN is an intrinsic inhibitor of progerin, abnormal splicing product of lamin A.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF, IP
Reactivity : Human
Huang YL et al. Hum Mol Genet 2015; 24 : 3506-17 FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169