Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C2] (GTX113296) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (GTX113296) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (GTX113296) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using 14-3-3 beta(GTX113296) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (20 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113296 diluted at 1:10000

14-3-3 beta antibody [N1C2] detects 14-3-3 beta protein at cytoplasm in rat cervix by immunohistochemical analysis.
Sample: Paraffin-embedded rat cervix.
14-3-3 beta antibody [N1C2] (GTX113296) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min