Five Pillars of Antibody Validation
GeneTex employs various analytic validation strategies to ensure both consistent quality and specificity.
Brochure - World Class Validated Antibodies
Download the latest version of GeneTex's World Class Validated Antibodies brochure.
GeneTex’s “beta-Amyloid (1-42) antibody – Conformation-Specific” can specifically detect amyloid plaques in the Alzheimer's Disease brain by IHC-P analysis. The superior specificity and sensitivity of this antibody were confirmed by dot blot, ELISA and side-by-side IHC-P comparison with competitor's.
Approach to Antibody Lot-to-Lot Variability
Lot-to-lot variability is a persistent quality control (QC) issue that contributes to inconsistent results in assays using antibodies.
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The validity of a target protein’s endogenous signal can be further substantiated through overexpression of that protein using a recombinant construct.
Detecting predicted changes in the antibody-generated signal due to alteration of experimental biological conditions is a reliable approach to gauge antibody performance.
Using mass spectrometry (MS) to identify the protein(s) bound by an antibody is a powerful method for the validation of antibody specificity and performance.
25μl package available!
Our most popular antibodies now come in 25μl packaging.
RNA interference (RNAi) refers to a cellular mechanism of post-transcriptional gene regulation through the actions of non-coding microRNAs (miRNAs).
Background of CRISPR/Cas9 technology
The RNA-guided CRISPR/Cas9 system is a revolutionary genome editing tool derived from the bacterial Type II CRISPR adaptive immune system.
Comparing the signals generated by two independent antibodies for the same protein target in different samples is a practical way to test antibody specificity.
GeneTex is conducting knockout/knockdown-based validation as a fundamental component of its antibody quality assurance process.
Wnt proteins are secreted morphogens that are required for various developmental processes.
Epithelial–mesenchymal transition (EMT) Pathway
EMT is a process whereby tightly-interacting and immotile epithelial cells acquire the phenotype of loosely-adherent and motile mesenchymal cells.
Protocol - Western Blot
Download GeneTex's Western Blot Protocol.
Protocol - ICC/IF
Download GeneTex's ICC/IF Protocol.
Protocol - IHC (paraffin)
Download GeneTex's IHC (paraffin) Protocol.
Protocol - IHC (frozen)
Download GeneTex's IHC (frozen) Protocol.
Protocol - IHC (whole-mount)
Download GeneTex's IHC (whole-mount) Protocol.
Protocol - Immunoprecipitation
Download GeneTex's Immunoprecipitation Protocol.
Protocol - ELISA (sandwich)
Download GeneTex's ELISA (sandwich) Protocol.
Protocol - ELISA (indirect)
Download GeneTex's ELISA (indirect) Protocol.
Protocol - Flow Cytometry
Download GeneTex's Flow Cytometry Protocol.
Protocol - Dot Blot
Download GeneTex's Dot Blot Protocol.
Protocol - Blocking Peptide Competition
Download GeneTex's Blocking Peptide Competition Protocol.
Flyer - Fluorescence Immunostaining
Download the latest version of GeneTex's fluorescence immunostaining flyer.