Five Pillars of Antibody Validation
GeneTex employs various analytic validation strategies to ensure both consistent quality and specificity.
GeneTex’s “beta-Amyloid (1-42) antibody – Conformation-Specific” can specifically detect amyloid plaques in the Alzheimer's Disease brain by IHC-P analysis. The superior specificity and sensitivity of this antibody were confirmed by dot blot, ELISA and side-by-side IHC-P comparison with competitor's.
Approach to Antibody Lot-to-Lot Variability
Lot-to-lot variability is a persistent quality control (QC) issue that contributes to inconsistent results in assays using antibodies.
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The validity of a target protein’s endogenous signal can be further substantiated through overexpression of that protein using a recombinant construct.
Detecting predicted changes in the antibody-generated signal due to alteration of experimental biological conditions is a reliable approach to gauge antibody performance.
Using mass spectrometry (MS) to identify the protein(s) bound by an antibody is a powerful method for the validation of antibody specificity and performance.
25μl package available!
Our most popular antibodies now come in 25μl packaging.
GeneTex is conducting knockout/knockdown-based validation as a fundamental component of its antibody quality assurance process.
Wnt proteins are secreted morphogens that are required for various developmental processes.
Protocol - Western Blot
Download GeneTex's Western Blot Protocol.
Protocol - ICC/IF
Download GeneTex's ICC/IF Protocol.
Protocol - IHC (paraffin)
Download GeneTex's IHC (paraffin) Protocol.
Protocol - IHC (frozen)
Download GeneTex's IHC (frozen) Protocol.
Protocol - IHC (whole-mount)
Download GeneTex's IHC (whole-mount) Protocol.
Protocol - Immunoprecipitation
Download GeneTex's Immunoprecipitation Protocol.
Protocol - ELISA (sandwich)
Download GeneTex's ELISA (sandwich) Protocol.
Protocol - ELISA (indirect)
Download GeneTex's ELISA (indirect) Protocol.
Protocol - Flow Cytometry
Download GeneTex's Flow Cytometry Protocol.
Protocol - Dot Blot
Download GeneTex's Dot Blot Protocol.
Protocol - Blocking Peptide Competition
Download GeneTex's Blocking Peptide Competition Protocol.
Flyer - Five Pillars Validation
Download the latest version of GeneTex's epitope five pillars validation flyer.
Flyer - Loading Controls
Download the latest version of GeneTex's loading controls flyer.
Pamphlet - Western Blot Troubleshooting
Download the latest version of GeneTex's pamphlet for western blot troubleshooting.
Flyer - Upgrade Your Western Blot Results
Download the latest version of GeneTex's flyer for upgrade your western blot results.
Flyer - IHC Troubleshooting Guide
Download the latest version of GeneTex's IHC troubleshooting guide flyer.
Flyer - Epitope Tags and Reporters
Download the latest version of GeneTex's epitope tags and reporters flyer.