Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C3] (GTX113297) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (20 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113297 diluted at 1:10000

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (GTX113297) diluted at 1:5000 and competitor's antibody (sc-628) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (GTX113297) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using 14-3-3 beta(GTX113297) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min