Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 gamma antibody (GTX113298) diluted at 1:1000 and competitor's antibody (SC-731) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113298 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 gamma antibody (GTX113298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma(GTX113298) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using 14-3-3 gamma(GTX113298) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

14-3-3 gamma antibody detects 14-3-3 gamma protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
14-3-3 gamma stained by 14-3-3 gamma antibody (GTX113298) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min