Sample (30 μg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX101075 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
12% SDS PAGE
GTX101075 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (GTX101075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using 14-3-3 zeta(GTX101075) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (GTX101075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
14-3-3 zeta antibody (GTX101075) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min