Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 15-PGDH antibody [C2C3], C-term (GTX104625) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using 15-PGDH(GTX104625) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX104625 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 15-PGDH(GTX104625) antibody at 1:200 dilution.