Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 15-PGDH antibody (GTX113740) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using 15-PGDH(GTX113740) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed A431, using 15-PGDH(GTX113740) antibody at 1:500 dilution.