ICC/IF analysis of HEK293 cells treated(B,D,F,H) or untreated(A,C,E,G) with 50 μM H₂O₂ using GTX175714 Hydroxynonenal antibody [12F7].
Green : Primary antibody
Red : F-Actin
Blue : DAPI
Dilution : 1:50 for 30-60 min at RT
Fixation : 5% Formaldehyde for 5 min

WB analysis of 4-hydroxy-nonenal-BSA using GTX175714 Hydroxynonenal antibody [12F7].
Lane 1 : Molecular Weight Ladder (MW)
Lane 2 : BSA (0.5 μg)
Lane 3 : 4-hydroxyl nonenal-BSA (0.5 μg)
Lane 4 : 4-hydroxy nonenal-BSA (2.0 μg)
Lane 5 : 4-hydroxy-2-hexenal (0.5 μg)
Lane 6 : 4-hydroxy-2-hexenal (2.0 μg)
Dilution : 1:1000 for 2 hours at RT