Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1E receptor antibody [HL3875] (GTX642113) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.