5-Methylcytosine / 5-mC antibody [GT4111]

Cat. No. GTX629448
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Cat. No.  GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629448
GTX629448 MeDIP Image

GTX629448 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (GTX629448) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

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Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448
GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

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Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.

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Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448
GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

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Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot blot (Dot). GTX629448
GTX629448 Dot Image

GTX629448 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

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Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot blot (Dot). GTX629448
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    GT4111
  • Isotype
    IgG1
  • Applications
    IHC-P IHC-Fr IHC-Wm Dot EM MeDIP
  • Reactivity
    Species independent
Summary
5-mC antibody detects methylated cytosine, which is a key epigenetic regulatory modification of genomic DNA and is considered a “fifth base”. The 5-mC mark is added by DNA methyltransferases (DNMTs) and serves predominately to turn off gene transcription. It is reversible via a DNA demethylation pathway triggered by the Ten-eleven translocation (TET) family of dioxygenases.
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