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5-Methylcytosine / 5-mC antibody [RM231]

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Methylated DNA Immunoprecipitation (MeDIP). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Flow cytometry (FACS). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Dot blot (Dot). GTX33606

Cat. No. GTX33606

Reference 2

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM231

Isotype

 IgG

Application

 ICC/IF, FACS, Dot, ELISA, IHC, MeDIP

Reactivity

 Species independent
Package
50 μg ($479)
See all 5-Methylcytosine / 5-mC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 0.5 μg/mL - 2 μg/mL
FACS 0.5 μg/mL - 2 μg/mL
Dot 0.5 μg/mL - 2 μg/mL
ELISA 0.1 μg/mL - 1 μg/mL
IHC Assay dependent
MeDIP 0.2 μg/mL - 2 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

BSA-conjugated 5-methylcytosine.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887725

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33606

GTX33606 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].
Red : Primary antibody
Green : Actin
Blue : DAPI

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606

GTX33606 ELISA Image

ELISA analysis of HeLa cell genomic DNA using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].
Dilution : 1 ug/mL or 3 ug/mL of RM231 was used as the primary antibody.

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Methylated DNA Immunoprecipitation (MeDIP). GTX33606

GTX33606 MeDIP Image

MeDIP analysis of 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) spiked in 1ug of genomic DNA isolated from HeLa cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231]. MeDIP was performed at a 10:1 DNA:Ab ratio. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606

GTX33606 ELISA Image

ELISA analysis of unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA using serial diluted GTX33606 5-Methylcytosine / 5-mC antibody [RM231].

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Flow cytometry (FACS). GTX33606

GTX33606 FACS Image

FACS analysis of HEK293 cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231]. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with GTX33606 (Blue) or with a negative control PEG antibody (Red).

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Dot blot (Dot). GTX33606

GTX33606 Dot Image

Dot blot analysis of 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].

REFERENCE

Application Reference

Kojima N et al. Biosens Bioelectron 2020; 167 : 112472 Quantitative analysis of global 5-methyl- and 5-hydroxymethylcytosine in TET1 expressed HEK293T cells.
Naoshi Kojima et al. Analytica Chimica Acta 2018; : 43108 Immobilization of DNA with nitrogen mustardebiotin conjugate for global epigenetic analysis.
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Package List Price ($)
$ 479