Western blot using GeneTex's affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag™ antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1,400.