Sample (30 ug of whole cell lysate)
A: U87-MG
B: MCF-7
7.5% SDS PAGE
GTX116235 diluted at 1:1000

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACCN4 antibody [N1N3] (GTX116235) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.