Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Untreated (–) and treated (+) VeroE6 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell and tissue extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right)

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: VeroE6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded dog stomach.
ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat colon.
ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.