Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AChE antibody [HL1102] detects AChE protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

AChE antibody [HL1102] detects AChE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AChE antibody [HL1102] detects AChE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AChE antibody [HL1102] detects AChE protein at dendrite by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).