WB analysis of various mouse tissue samples using GTX00690 ADAM1B antibody [#158]. The Adam1B synthesized in testis (120 kDa) is proteolytically processed into smaller form (60 kDa) in sperm during the epididymal maturation of the spermatozoa. By western blotting, ADAM1B is detected in testis extract at 120 kDa position, which is due to glycosylation and larger than predicted size of 89 kDa from the sequence.
This protien is testis-specific.
Dilution : 1:100

ICC/IF analysis of fresh mouse sperms using GTX00690 ADAM1B antibody [#158].
Dilution : 1:300
Fixation : 4% PFA

WB analysis of mouse testis lysates collected from different ages using GTX00690 ADAM1B antibody [#158].
ADAM1B appeared as a faint signal at 3 weeks of age.