Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (GTX102835) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (GTX102835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AF9 antibody detects AF9 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AF9 stained by AF9 antibody (GTX102835) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AF9 protein stained by AF9 antibody (GTX102835) diluted at 1:1000.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

AF9 antibody detects AF9 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
AF9 stained by AF9 antibody (GTX102835) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using AF9(GTX102835) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (GTX102835) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.