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AIF antibody [4E7]

Anti-AIF antibody [4E7] used in Western Blot (WB). GTX60476
Anti-AIF antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX60476
Anti-AIF antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX60476
Anti-AIF antibody [4E7] used in Flow cytometry (FACS). GTX60476
Anti-AIF antibody [4E7] used in ELISA (ELISA). GTX60476
Anti-AIF antibody [4E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60476
Anti-AIF antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX60476
Anti-AIF antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX60476

Cat. No. GTX60476

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E7

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($409)
See all AIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human AIF expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887972

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apoptosis inducing factor mitochondria associated 1 , AIF , CMT2D , CMTX4 , COWCK , COXPD6 , DFNX5 , NADMR , NAMSD , PDCD8

Cellular Localization

Mitochondrion inner membrane,Cytoplasm,Nucleus

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-AIF antibody [4E7] used in Western Blot (WB). GTX60476

GTX60476 WB Image

WB analysis of NIH3T3 (1), Jurkat (2), HeLa (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate using GTX60476 AIF antibody [4E7].

Anti-AIF antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX60476

GTX60476 IHC-P Image

IHC-P analysis of human rectum cancer tissue using GTX60476 AIF antibody [4E7].

Anti-AIF antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX60476

GTX60476 IHC-P Image

IHC-P analysis of human cervical cancer tissue using GTX60476 AIF antibody [4E7].

Anti-AIF antibody [4E7] used in Flow cytometry (FACS). GTX60476

GTX60476 FACS Image

FACS analysis of HepG2 cells using GTX60476 AIF antibody [4E7].
Blue : AIF
Red : negative control

Anti-AIF antibody [4E7] used in ELISA (ELISA). GTX60476

GTX60476 ELISA Image

ELISA analysis of antigen using GTX60476 AIF antibody [4E7].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-AIF antibody [4E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60476

GTX60476 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX60476 AIF antibody [4E7].
Green : AIF
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-AIF antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX60476

GTX60476 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX60476 AIF antibody [4E7].

Anti-AIF antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX60476

GTX60476 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX60476 AIF antibody [4E7].

REFERENCE

Application Reference

Huang C et al. Pharmaceuticals (Basel) 2023; 16 (5) α-Viniferin-Induced Apoptosis through Downregulation of SIRT1 in Non-Small Cell Lung Cancer Cells.
Shen H et al. Front Aging Neurosci 2018; 10 : 353 Age-Dependent Up-Regulation of HCN Channels in Spiral Ganglion Neurons Coincide With Hearing Loss in Mice.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409