Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term (GTX102399) diluted at 1:4000.

Immunohistochemical analysis of paraffin-embedded human lung cancer, using AIF(GTX102399) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AIF(GTX102399) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
AIF antibody [N1N2], N-term (GTX102399) dilution: 1:1000

AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: AIFM1 protein stained by AIF antibody [N1N2], N-term (GTX102399) diluted at 1:500.
Blue: Hoechst 33343 staining.