Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AKAP12 antibody [C3] (GTX106373) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AKAP12 antibody [C3], C-term (GTX106373) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12(GTX106373) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using AKAP12(GTX106373) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min