Wild-type (WT) and AKR1B1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1B1 antibody [N1C3] (GTX113381) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using AKR1B1(GTX113381) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKR1B1(GTX113381) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1B1 antibody [N1C3] (GTX113381) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

The data was published in the journal Antioxidants (Basel) in 2020.PMID: 32218152