ICC/IF analysis of formalin-fixed Jurkat cells using GTX54911 AKR1B1 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

WB analysis of Jurkat (A), HeLa (B), SP2/0 (C), PC12 (D) whole cell lysates using GTX54911 AKR1B1 antibody.

IHC-P analysis of formalin fixed human colon cancer tissue section using GTX54911 AKR1B1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)