Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with AKR1B10 antibody (GTX109571) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AKR1B10 antibody detects AKR1B10 protein by western blot analysis.
A. 50 μg mouse colon lysate/extract
10% SDS-PAGE
AKR1B10 antibody (GTX109571) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10(GTX109571) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10(GTX109571) antibody at 1:200 dilution.

AKR1B10 antibody detects AKR1B10 protein by western blot analysis.
A. 50 μg rat colon lysate/extract
10% SDS-PAGE
AKR1B10 antibody (GTX109571) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.