Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1C3(GTX104627) antibody at 1:200 dilution.

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX104627 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 μg of AKR1C3 antibody [C2C3], C-term (GTX104627).
Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term (GTX104627).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using AKR1C3(GTX104627) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min