Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat esophagus.
AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AKT antibody [HL2915] detects AKT protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.