APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human AKT.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AKT serine/threonine kinase 1 , AKT serine/threonine kinase 2 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2
Cellular Localization
Cytoplasm,Nucleus,Cell membrane,Early endosome,Membrane
Database
Research Area
DATA IMAGES
![Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258](/templates/default/js/lightbox/images/loading.gif "Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258")
|
GTX640258 IHC-P Image
AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX640258 IHC-P Image
AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX640258 ICC/IF Image
AKT antibody [HL2915] detects AKT protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX640258 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX640258 IHC-P Image
AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded rat esophagus.AKT stained by AKT antibody [HL2915] (GTX640258) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX640258 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX640258 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX640258 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (GTX640258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for AKT antibody [HL2915] (GTX640258). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for AKT antibody [HL2915] (GTX640258). Be the first to share your experience with this product.
![Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258](/upload/website/prouct_img/small/GTX640258/GTX640258_T-45390_20240619_IHC-P_24062501_700.webp "Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258")
![Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258](/upload/website/prouct_img/small/GTX640258/GTX640258_T-45390_20240619_IHC-P_multiple_M_24062501_370.webp "Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258")
![Anti-AKT antibody [HL2915] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640258](/upload/website/prouct_img/small/GTX640258/GTX640258_T-45390_20240510_ICC_IF_24052202_426.webp "Anti-AKT antibody [HL2915] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640258")
![Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258](/upload/website/prouct_img/small/GTX640258/GTX640258_T-45390_20240531_WB_24060619_187.webp "Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258")
![Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258](/upload/website/prouct_img/small/GTX640258/GTX640258_T-45390_20240619_IHC-P_R_24062501_570.webp "Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258")
![Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258](/upload/website/prouct_img/small/GTX640258/GTX640258_T-45390_20240503_WB_R_24050701_127.webp "Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258")
![Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258](/upload/website/prouct_img/small/GTX640258/GTX640258_T-45390_20240503_WB_multiple_B_24050701_378.webp "Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258")
![Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258](/upload/website/prouct_img/small/GTX640258/GTX640258_T-45390_20240503_WB_M_24050701_791.webp "Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258")
![Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258](/upload/website/prouct_img/normal/GTX640258/GTX640258_T-45390_20240619_IHC-P_24062501_700.webp "Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258")
![Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258](/upload/website/prouct_img/normal/GTX640258/GTX640258_T-45390_20240619_IHC-P_multiple_M_24062501_370.webp "Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258")
![Anti-AKT antibody [HL2915] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640258](/upload/website/prouct_img/normal/GTX640258/GTX640258_T-45390_20240510_ICC_IF_24052202_426.webp "Anti-AKT antibody [HL2915] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640258")
![Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258](/upload/website/prouct_img/normal/GTX640258/GTX640258_T-45390_20240531_WB_24060619_187.webp "Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258")
![Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258](/upload/website/prouct_img/normal/GTX640258/GTX640258_T-45390_20240619_IHC-P_R_24062501_570.webp "Anti-AKT antibody [HL2915] used in IHC (Paraffin sections) (IHC-P). GTX640258")
![Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258](/upload/website/prouct_img/normal/GTX640258/GTX640258_T-45390_20240503_WB_R_24050701_127.webp "Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258")
![Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258](/upload/website/prouct_img/normal/GTX640258/GTX640258_T-45390_20240503_WB_multiple_B_24050701_378.webp "Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258")
![Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258](/upload/website/prouct_img/normal/GTX640258/GTX640258_T-45390_20240503_WB_M_24050701_791.webp "Anti-AKT antibody [HL2915] used in Western Blot (WB). GTX640258")