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AKT antibody [N2C2], Internal

Anti-AKT antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121936
Anti-AKT antibody [N2C2], Internal used in Immunohistochemistry (IHC). GTX121936
Anti-AKT antibody [N2C2], Internal used in Western Blot (WB). GTX121936

Cat. No. GTX121936

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AKT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HA-human Akt -transfected 293T cells

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Akt1/2/3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721691

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT antibody , MGC99656 antibody , PKB antibody , PKB-ALPHA antibody , PRKBA antibody , RAC antibody , RAC-ALPHA antibody , AKT1 antibody , protein kinase B alpha antibody , rac protein kinase alpha antibody , RAC-alpha serine/threonine-protein kinase antibody , proto-oncogene c-Akt antibody , RAC-PK-alpha antibody , PKB alpha antibody , v-akt murine thymoma viral oncogene homolog 1 antibody , PKBB antibody , PKBBETA antibody , PRKBB antibody , RAC-BETA antibody , AKT2 antibody , protein kinase B beta antibody , murine thymoma viral (v-akt) homolog-2 antibody , protein kinase Akt-2 antibody , RAC-PK-beta antibody , PKB beta antibody , rac protein kinase beta antibody , RAC-beta serine/threonine-protein kinase antibody , v-akt murine thymoma viral oncogene homolog 2 antibody , DKFZp434N0250 antibody , PKB-GAMMA antibody , PKBG antibody , PRKBG antibody , RAC-PK-gamma antibody , RAC-gamma antibody , STK-2 antibody , AKT3 antibody , PKB gamma antibody , RAC-gamma serine/threonine-protein kinase antibody , RAC-gamma serine/threonine protein kinase antibody , v-akt murine thymoma viral oncogene homolog 3 (protein kinase B , gamma) antibody

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-AKT antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121936

GTX121936 ICC/IF Image

Akt1/2/3 antibody detects Akt1/2/3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Akt1 protein stained by Akt1/2/3 antibody (GTX121936) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-AKT antibody [N2C2], Internal used in Immunohistochemistry (IHC). GTX121936

GTX121936 IHC Image

Staining of Human esophageal cancer tissue sections using anti-Akt1/2/3 antibody (GTX121936).

Anti-AKT antibody [N2C2], Internal used in Western Blot (WB). GTX121936

GTX121936 WB Image

Akt1/2/3 antibody detects Akt1/2/3 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of HA-human Akt-transfected 293T cells
7.5 % SDS-PAGE
Akt1/2/3 antibody (GTX121936) dilution: 1:5000

REFERENCE

Application Reference

Wang L et al. Bioengineered 2022; 13 (5) : 12941-12954 Stearoyl-CoA desaturase 1 regulates malignant progression of cervical cancer cells.
Liu J et al. Mol Med Rep 2019; 20 (1) : 33-40 miR?338?3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β?catenin signaling pathways.
Application : WB
Reactivity : Human
Bakhit A et al. Sci Rep 2018; 8 (1) : 9224 Strontium ranelate promotes odonto-/osteogenic differentiation/mineralization of dental papillae cells in vitro and mineralized tissue formation of the dental pulp in vivo.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169