Sample (30 μg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX121937 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 μg of Akt1/2/3 antibody [N3C2], Internal (GTX121937).
Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GTX121937).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

AKT antibody [N3C2], Internal detects AKT protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AKT antibody [N3C2], Internal detects AKT protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal Sci Rep in 2017.PMID: 29038536

The data was published in the journal PLoS One in 2017.PMID: 28591208

The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851

The data was published in the journal Sci Rep in 2017.PMID: 29038536

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

The data was published in the journal Oncogene in 2018. PMID: 29993037

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

The data was published in the journal Theranostics in 2019. PMID: 31754399

The data was published in the journal Cell Death Dis in 2014. PMID: 24457964

The data was published in the journal Biomed Res Int in 2015. PMID: 26557697

The data was published in the journal Nutrients in 2018.PMID: 30200495

The data was published in the journal Sci Rep in 2017.PMID: 29038536

The data was published in the journal Sci Rep in 2017.PMID: 29038536

The data was published in the 2022 in Front Genet. PMID: 35401669

The data was published in the 2022 in Nat Commun. PMID: 35169117