Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA-human Akt1-transfected 293T cells
C. 5 μg whole cell lysate/extract of HA-human Akt2-transfected 293T cells
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:20000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:500

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg Raw264.7 whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:500

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.