Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ALAS-E antibody [N3C3]  (GTX104139) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX104139 diluted at 1:1000

The data was published in the journal Front Pharmacol in 2014. PMID: 24860503