ALDH1A1 antibody [C3-2], C-term

Name: ALDH1A1 antibody [C3-2], C-term
Brand: GeneTex
SKU: GTX100044
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-ALDH1A1 antibody [C3-2], C-term used in Western Blot (WB). GTX100044

Anti-ALDH1A1 antibody [C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX100044

Cat. No. GTX100044

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all ALDH1A1 products

Datasheet
Document

View product citations for antibody GTX100044 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Sheep, Bovine, Dog, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ALDH1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1080929

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member A1 , ALDC , ALDH-E1 , ALDH1 , ALDH11 , HEL-9 , HEL-S-53e , HEL12 , PUMB1 , RALDH1

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Database

Gene ID: 216 ALDH1A1
UniProt: P00352 ALDH1A1

Research Area

DATA IMAGES

GTX100044 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX100044 diluted at 1:1000

GTX100044 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DU-145 xenograft, using ALDH1A1(GTX100044) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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REVIEW