Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
ALDH7A1 antibody
GTX114274 diluted at 1:1000

ALDH7A1 antibody [C1C3] detects ALDH7A1 protein at mitochondria and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: ALDH7A1 protein stained by ALDH7A1 antibody [C1C3] (GTX114274) diluted at 1:500.
Blue: Hoechst 33342 staining.

ALDH7A1 antibody [C1C3] detects ALDH7A1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ALDH7A1 antibody [C1C3] (GTX114274) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX114274 diluted at 1:10000

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH7A1 antibody [C1C3] (GTX114274) diluted at 1:3000.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH7A1 antibody [C1C3] (GTX114274) at 1:300 dilution.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX114274 diluted at 1:1000