Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ALY(GTX113917) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ALY antibody (GTX113917) diluted at 1:500.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113917 diluted at 1:1000

ALY antibody detects ALY protein at nucleus in human esophageal cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal cancer.
ALY antibody (GTX113917) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ALY antibody detects ALY protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ALY antibody (GTX113917) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min