HepG2 conditioned medium (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ANGPTL3 antibody (GTX104569) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ANGPTL3(GTX104569) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ANGPTL3 antibody detects ANGPTL3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
ANGPTL3 stained by ANGPTL3 antibody (GTX104569) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min