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ANKRD26 antibody

Anti-ANKRD26 antibody used in Western Blot (WB). GTX128255
Anti-ANKRD26 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128255
Anti-ANKRD26 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128255
Anti-ANKRD26 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128255

Cat. No. GTX128255

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ANKRD26 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

196 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ANKRD26. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885741

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ankyrin repeat domain 26 , THC2 , bA145E8.1

Background

This gene encodes a protein containing N-terminal ankyrin repeats which function in protein-protein interactions. Mutations in this gene are associated with autosomal dominant thrombocytopenia-2. Pseudogenes of this gene are found on chromosome 7, 10, 13 and 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-ANKRD26 antibody used in Western Blot (WB). GTX128255

GTX128255 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ANKRD26 antibody (GTX128255) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ANKRD26 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128255

GTX128255 ICC/IF Image

The data was published in the journal Nat Commun in 2020.PMID: 32366837

Anti-ANKRD26 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128255

GTX128255 ICC/IF Image

The data was published in the journal Nat Commun in 2019.PMID: 30824690

Anti-ANKRD26 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128255

GTX128255 ICC/IF Image

The data was published in the journal Nat Commun in 2019.PMID: 30824690

REFERENCE

Application Reference

Jiang YZ et al. World J Stem Cells 2024; 16 (5) : 538-550 GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines.
Curinha A et al. bioRxiv 2024; Centriole structural integrity defects are a crucial feature of Hydrolethalus Syndrome.
Takeda Y et al. Nat Commun 2024; 15 (1) : 2216 Molecular basis promoting centriole triplet microtubule assembly.
S Carden et al. Dev Cell 2023; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method
Application : ICC/IF
Hall EA et al. Elife 2023; 12 Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis.
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169