Sandwich ELISA detection of recombinant Human ANP protein, GST tag (GTX109255-pro) using antibodies as below.

Capture: ANP antibody [GT1068] (GTX634219) (5 μg/mL)
Detection: ANP antibody [HL2617] (GTX639071) (1 μg/mL)

ANP antibody [GT1068] detects secreted ANP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ANP antibody [GT1068] detects ANP protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [GT1068] (GTX634219) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.