Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody (GTX109255) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ANP antibody detects ANP protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
ANP antibody (GTX109255) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ANP antibody detects ANP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ANP stained by ANP antibody (GTX109255) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ANP antibody detects ANP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
ANP stained by ANP antibody (GTX109255) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal Sci Rep in 2019. PMID: 30816171

The data was published in the journal PLoS One in 2015.PMID: 25679215