Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX114832 diluted at 1:1000

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AP2 beta antibody (GTX114832) diluted at 1:500.

AP2 beta antibody immunoprecipitates AP2 beta protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. 30 μg MCF-7 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of AP2 beta protein by 4 μg AP2 beta antibody (GTX114832)
7.5 % SDS-PAGE
The immunoprecipitated AP2 beta protein was detected by AP2 beta antibody (GTX114832) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]