Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX107122) diluted at 1:3000.

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX107122 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX107122 diluted at 1:3000

Immunohistochemical analysis of paraffin-embedded human lung cancer, using APE1(GTX107122) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min