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APP (phospho Thr668) antibody

Orthogonal
Anti-APP (phospho Thr668) antibody used in Western Blot (WB). GTX50235
Anti-APP (phospho Thr668) antibody used in Western Blot (WB). GTX50235

Cat. No. GTX50235

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all APP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 668 (A-V-T(p)-P-E) derived from human APP.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11175236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4

Cellular Localization

Cell membrane,Membrane,Cytoplasmic vesicle,Cytoplasm

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

Database

Research Area

DATA IMAGES

Anti-APP (phospho Thr668) antibody used in Western Blot (WB). GTX50235

GTX50235 WB Image

WB analysis of extracts from mouse brain tissue using GTX50235 APP (phospho Thr668) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

Anti-APP (phospho Thr668) antibody used in Western Blot (WB). GTX50235

GTX50235 WB Image

Orthogonal

WB analysis of extracts from HeLa cells treated with Noc or calf intestinal phosphatase (CIP) using GTX50235 APP (phospho Thr668) antibody.

REFERENCE

Application Reference

Bukhari H et al. Cell Signal 2016; 28 (11) : 1725-34 Membrane tethering of APP c-terminal fragments is a prerequisite for T668 phosphorylation preventing nuclear sphere generation.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399