ICC/IF analysis of U251 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

FACS analysis of MCF-7 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue).
Dilution : 1:80 for 60 min at room temperature
Fixation : 2% paraformaldehyde

IHC-P analysis of human colon carcinoma tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

ICC/IF analysis of HeLa cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

FACS analysis of 3T3 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue).
Dilution : 1:80 for 60 min at room temperature
Fixation : 2% paraformaldehyde

WB analysis of canine heart extract using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].

IHC-P analysis of human liver tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

IHC-P analysis of human tonsil tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

ICC/IF analysis of MCF-7 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

FACS analysis of Hela cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue).
Dilution : 1:80 for 60 min at room temperature
Fixation : 2% paraformaldehyde