Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Arginase II(GTX104036) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

293T whole cell and cytoplasm extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody (GTX104036) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody (GTX104036) diluted at 1:1000.

ARG2 antibody detects ARG2 protein at mitochondria in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ARG2 antibody (GTX104036) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ARG2 antibody detects ARG2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ARG2 antibody (GTX104036) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Arginase II antibody detects ARG2 protein by western blot analysis.
A. 50 μg mouse kidney lysate/extract
10% SDS-PAGE
Arginase II antibody (GTX104036) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Neurotherapeutics in 2018. PMID: 30288668