ARID1A antibody immunoprecipitates ARID1A protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ARID1A protein by 4 μg ARID1A antibody (GTX129433)
5 % SDS-PAGE
The immunoprecipitated ARID1A protein was detected by ARID1A antibody (GTX129433) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

ARID1A antibody detects ARID1A protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ARID1A antibody (GTX129433) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ARID1A antibody detects ARID1A protein by Western blot analysis.
A. 30 μg HepG2 whole cell extract
B. 30 μg HepG2 nuclear extract
5 % SDS-PAGE
ARID1A antibody (GTX129433) dilution: 1:1000

ARID1A antibody detects ARID1A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody (GTX129433) diluted by 1:1000.

ARID1A antibody detects ARID1A protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
ARID1A antibody (GTX129433) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ARID1A antibody detects ARID1A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ARID1A stained by ARID1A antibody (GTX129433) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ARID1A antibody detects ARID1A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID1A stained by ARID1A antibody (GTX129433) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody (GTX129433) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Leukemia in 2019. PMID: 30858552