Name: ASFV p54 antibody [GT853]
Brand: GeneTex
SKU: GTX635690
Price: 419.00 USD
Rating: 5 (1 reviews)

ASFV p54 antibody [GT853]

Cat. No. GTX635690

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Cat. No. GTX635690

GTX635690 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635690 [GT853])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

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GTX635690 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635690 [GT853])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

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GTX635690 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [GT853] (GTX635690) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX635690 ICC/IF Image

ASFV p54 antibody [GT853] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [GT853] (GTX635690) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

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GTX635690 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro, 0.2 μg/ml) by lateral flow assay.

Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 μg/ml). Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 μg/ml).

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GTX635690 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture:
ASFV p54 antibody [GT853] (GTX635690) (5 μg/mL)
Detection:
HRP-conjugated ASFV p54 antibody [GT1075] (GTX635691) (1 μg/mL)
Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.

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GTX635690 IHC-P Image

ASFV p54 antibody [GT853] detects ASFV p54 protein at cytoplasm by immunohistochemical analysis.
Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block.
Green: ASFV p54 stained by ASFV p54 antibody [GT853] (GTX635690) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX635690 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [GT853] (GTX635690) (5 μg/mL)
Detection: ASFV p54 antibody (GTX135175) (1 μg/mL).

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Host

Mouse

Clonality

Monoclonal

Clone Name

GT853

Isotype

IgG1

Applications

WB ICC/IF IHC-P ELISA Lateral Flow Sandwich ELISA

Reactivity

African swine fever virus

Summary

ASFV p54 antibody detects envelope protein p54 of African swine fever virus. Protein p54, encoded by the E183L gene, is a 20 kDa type I transmembrane protein that localizes to envelope precursors derived from the host ER. p54 is involved in intracellular microtubule-dependent transport of viral capsid toward viral factories, and is detected on membrane-like structures within viral factories and mature extracellular virions. This is a valuable mouse monoclonal antibody for research into this very contagious hemorrhagic disease of pigs.

See all ASFV p54 products