Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636563 [HL1219])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (20 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636563 [HL1219])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (20 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1219] (GTX636563) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant recombinant HEK293 expressed, full-length ASFV p54 (40-0.63 nM). Coated protein was probed with ASFV p54 antibody [HL1219] (GTX636563) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1218] (GTX636562) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1219] (GTX636563) (0.1 μg/mL)
Please notice that GTX636563 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636562. Please contact us for custom HRP-conjugated antibody.

ASFV p54 antibody [HL1219] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [HL1219] (GTX636563) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.