Unboiled SK-N-AS whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ASIC1 antibody [GT73] (GTX641718) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)